upright nikon a1r confocal microscope (Nikon)
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Upright Nikon A1r Confocal Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 63936 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 63936 article reviews
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Fluorescence:Article Title: MSL10 is a high-sensitivity mechanosensor in the tactile sense of the Venus flytrap Article Snippet: Leaf blades from the GCaMP6f overexpression line were cut using a razor blade, placed on a glass slide (Fisher Scientific), and immersed in extracellular fluid (ECF; containing 0.1 mM KCl [Wako], 10 mM CaCl 2 [Wako], 20 mM sorbitol [Wako] and 5 mM 2-(N-morpholino)ethanesulfonic acid [Dojindo], adjusted to pH 6.0 with 2-amino-2-hydroxymethyl-1,3-propanediol (Tris) [Wako]) , . .. GCaMP6f fluorescence was observed on the adaxial and abaxial sides of the leaf blades using an Article Title: MSL10 is a high-sensitivity mechanosensor in the tactile sense of the Venus flytrap. Article Snippet: Confocal imaging of the leaf blades Leaf blades from the GCaMP6f overexpression line were cut using a razor blade, placed on a glass slide (Fisher Scientific), and immersed in extracellular fluid (ECF; containing 0.1mM KCl [Wako], 10mM CaCl2 [Wako], 20mM sorbitol [Wako] and 5mM 2-(N-morpholino)ethanesulfonic acid [Dojindo], adjusted to pH 6.0 with 2-amino-2-hydroxymethyl-1,3-propanediol (Tris) [Wako])6,40. .. GCaMP6f fluorescence was observed on the adaxial and abaxial sides of the leaf blades using an Microscopy:Article Title: MSL10 is a high-sensitivity mechanosensor in the tactile sense of the Venus flytrap Article Snippet: Leaf blades from the GCaMP6f overexpression line were cut using a razor blade, placed on a glass slide (Fisher Scientific), and immersed in extracellular fluid (ECF; containing 0.1 mM KCl [Wako], 10 mM CaCl 2 [Wako], 20 mM sorbitol [Wako] and 5 mM 2-(N-morpholino)ethanesulfonic acid [Dojindo], adjusted to pH 6.0 with 2-amino-2-hydroxymethyl-1,3-propanediol (Tris) [Wako]) , . .. GCaMP6f fluorescence was observed on the adaxial and abaxial sides of the leaf blades using an Article Title: MSL10 is a high-sensitivity mechanosensor in the tactile sense of the Venus flytrap. Article Snippet: Confocal imaging of the leaf blades Leaf blades from the GCaMP6f overexpression line were cut using a razor blade, placed on a glass slide (Fisher Scientific), and immersed in extracellular fluid (ECF; containing 0.1mM KCl [Wako], 10mM CaCl2 [Wako], 20mM sorbitol [Wako] and 5mM 2-(N-morpholino)ethanesulfonic acid [Dojindo], adjusted to pH 6.0 with 2-amino-2-hydroxymethyl-1,3-propanediol (Tris) [Wako])6,40. .. GCaMP6f fluorescence was observed on the adaxial and abaxial sides of the leaf blades using an Article Title: MSL10 is a high-sensitivity mechanosensor in the tactile sense of the Venus flytrap Article Snippet: .. The sections were observed under an Article Title: A novel protein-preserving passive tissue clearing approach using sodium cholate and urea for whole-organ imaging. Article Snippet: .. The imaging was performed using an Article Title: In vivo programming of adult pericytes aids axon regeneration by providing cellular bridges for SCI repair. Article Snippet: 872 873 Calcium imaging 874 The membrane-permeable calcium indicator Fluo-4 AM (F14201, Invitrogen) was added 875 to the cell culture medium (final concentration: 5 μM) and kept at 36.5°C in the cell culture 876 incubator for 45-60 minutes. .. The coverslip was then transferred to a submerged imaging 877 chamber mounted on a Article Title: Early life ACE2 inhibition leads to long term anxiety-like behaviour in rats by disrupting the amygdala. Article Snippet: .. To assess the number of microglia, Iba1 images were taken on an Article Title: MSL10 is a high-sensitivity mechanosensor in the tactile sense of the Venus flytrap. Article Snippet: .. The sections were observed under an Article Title: A novel protein-preserving passive tissue clearing approach using sodium cholate and urea for whole-organ imaging Article Snippet: .. The imaging was performed using an Imaging:Article Title: A novel protein-preserving passive tissue clearing approach using sodium cholate and urea for whole-organ imaging. Article Snippet: .. The imaging was performed using an Article Title: In vivo programming of adult pericytes aids axon regeneration by providing cellular bridges for SCI repair. Article Snippet: 872 873 Calcium imaging 874 The membrane-permeable calcium indicator Fluo-4 AM (F14201, Invitrogen) was added 875 to the cell culture medium (final concentration: 5 μM) and kept at 36.5°C in the cell culture 876 incubator for 45-60 minutes. .. The coverslip was then transferred to a submerged imaging 877 chamber mounted on a Article Title: A novel protein-preserving passive tissue clearing approach using sodium cholate and urea for whole-organ imaging Article Snippet: .. The imaging was performed using an Software:Article Title: A novel protein-preserving passive tissue clearing approach using sodium cholate and urea for whole-organ imaging. Article Snippet: .. The imaging was performed using an Article Title: A novel protein-preserving passive tissue clearing approach using sodium cholate and urea for whole-organ imaging Article Snippet: .. The imaging was performed using an |
![( A to H ) Migration and deformation of endogenous murine neutrophils 2 hours after N. meningitidis local skin injection. (A) <t>Spinning-disk</t> confocal images of skin capillaries with neutrophils indicated by arrowheads. BSA, bovine serum albumin. (B) Capillary segments with different diameters. (C) Frequency distribution of capillary diameters ( n = 139) and round neutrophils in large vessels ( n = 67). (D) Neutrophil confinement in capillaries. Neutrophils shown in orange. (E) Montage of 30 cells pseudocolored by the value of their AR. (F) Individual mean cell AR and (G) mean cell speed ( n = 61 cells). (H) Scatterplot comparing instantaneous speed and instantaneous cell AR ( n = 679 time points from 61 cells). ( I to L ) Mature mNeus migrating in microchannels of different widths. (I) Representative widefield microscopy images of individual mNeus (Hoechst, magenta) in each channel size. (J) Distribution of mean cell AR, n > 150 cells per channel size, and (K) mean cell speed, n > 250 cells per channel size. (L) Scatterplot comparing instantaneous speed and instantaneous cell AR of mNeus in 7-, 5-, and 3-μm channel widths. Data are one representative of three independent experiments; n = 2189 (7 μm), n = 2876 (5 μm), and n = 2137 (3 μm). Data are pooled from [(C) and (F) to (H)] three or (K) five independent experiments. Data are displayed as [(F) and (G)] median by a line or [(C), (J), and (K)] frequency distributions (percentage) with indicated means ± SD. Each dot represents one cell. Data were analyzed by [(H) and (L)] Spearman correlation test (two-tailed). Scale bars, [(B), (D), and (I)] 5 μm, (E) 10 μm, and (A) 20 μm.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_6789/pmc13676789/pmc13676789__nihms-2212406-f0001.jpg)