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upright nikon a1r confocal microscope  (Nikon)


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    Structured Review

    Nikon upright nikon a1r confocal microscope
    Upright Nikon A1r Confocal Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 63936 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/upright+confocal+microscope/Objectives/pm41903542-370-6-7
    Average 99 stars, based on 63936 article reviews
    upright nikon a1r confocal microscope - by Bioz Stars, 2026-11
    99/100 stars

    Images

    Related Articles

    Fluorescence:

    Article Title: MSL10 is a high-sensitivity mechanosensor in the tactile sense of the Venus flytrap
    Article Snippet: Leaf blades from the GCaMP6f overexpression line were cut using a razor blade, placed on a glass slide (Fisher Scientific), and immersed in extracellular fluid (ECF; containing 0.1 mM KCl [Wako], 10 mM CaCl 2 [Wako], 20 mM sorbitol [Wako] and 5 mM 2-(N-morpholino)ethanesulfonic acid [Dojindo], adjusted to pH 6.0 with 2-amino-2-hydroxymethyl-1,3-propanediol (Tris) [Wako]) , . .. GCaMP6f fluorescence was observed on the adaxial and abaxial sides of the leaf blades using an upright confocal microscope (A1R, Nikon) equipped with a 20× objective lens (S Plan Fluor ELWD 20XC, Nikon) and an inverted confocal microscope (A1R, Nikon) equipped with a 20× objective lens (Plan Apo VC 20x DIC N2, Nikon). ..

    Article Title: MSL10 is a high-sensitivity mechanosensor in the tactile sense of the Venus flytrap.
    Article Snippet: Confocal imaging of the leaf blades Leaf blades from the GCaMP6f overexpression line were cut using a razor blade, placed on a glass slide (Fisher Scientific), and immersed in extracellular fluid (ECF; containing 0.1mM KCl [Wako], 10mM CaCl2 [Wako], 20mM sorbitol [Wako] and 5mM 2-(N-morpholino)ethanesulfonic acid [Dojindo], adjusted to pH 6.0 with 2-amino-2-hydroxymethyl-1,3-propanediol (Tris) [Wako])6,40. .. GCaMP6f fluorescence was observed on the adaxial and abaxial sides of the leaf blades using an upright confocal microscope (A1R, Nikon) equipped with a 20× objective lens (S Plan Fluor ELWD 20XC, Nikon) and an inverted confocalmicroscope (A1R,Nikon) equippedwith a 20×objective lens (Plan Apo VC 20xDICN2, Nikon). ..

    Microscopy:

    Article Title: MSL10 is a high-sensitivity mechanosensor in the tactile sense of the Venus flytrap
    Article Snippet: Leaf blades from the GCaMP6f overexpression line were cut using a razor blade, placed on a glass slide (Fisher Scientific), and immersed in extracellular fluid (ECF; containing 0.1 mM KCl [Wako], 10 mM CaCl 2 [Wako], 20 mM sorbitol [Wako] and 5 mM 2-(N-morpholino)ethanesulfonic acid [Dojindo], adjusted to pH 6.0 with 2-amino-2-hydroxymethyl-1,3-propanediol (Tris) [Wako]) , . .. GCaMP6f fluorescence was observed on the adaxial and abaxial sides of the leaf blades using an upright confocal microscope (A1R, Nikon) equipped with a 20× objective lens (S Plan Fluor ELWD 20XC, Nikon) and an inverted confocal microscope (A1R, Nikon) equipped with a 20× objective lens (Plan Apo VC 20x DIC N2, Nikon). ..

    Article Title: MSL10 is a high-sensitivity mechanosensor in the tactile sense of the Venus flytrap.
    Article Snippet: Confocal imaging of the leaf blades Leaf blades from the GCaMP6f overexpression line were cut using a razor blade, placed on a glass slide (Fisher Scientific), and immersed in extracellular fluid (ECF; containing 0.1mM KCl [Wako], 10mM CaCl2 [Wako], 20mM sorbitol [Wako] and 5mM 2-(N-morpholino)ethanesulfonic acid [Dojindo], adjusted to pH 6.0 with 2-amino-2-hydroxymethyl-1,3-propanediol (Tris) [Wako])6,40. .. GCaMP6f fluorescence was observed on the adaxial and abaxial sides of the leaf blades using an upright confocal microscope (A1R, Nikon) equipped with a 20× objective lens (S Plan Fluor ELWD 20XC, Nikon) and an inverted confocalmicroscope (A1R,Nikon) equippedwith a 20×objective lens (Plan Apo VC 20xDICN2, Nikon). ..

    Article Title: MSL10 is a high-sensitivity mechanosensor in the tactile sense of the Venus flytrap
    Article Snippet: .. The sections were observed under an upright confocal microscope (A1R, Nikon) equipped with a 4× objective lens (Plan Fluor 4X, Nikon). ..

    Article Title: A novel protein-preserving passive tissue clearing approach using sodium cholate and urea for whole-organ imaging.
    Article Snippet: .. The imaging was performed using an upright confocal microscope (C2Si, Nikon) with a Plan-Apochromat 10× objective lens (numerical aperture (NA) 0.5 and working distance (WD) 5.5 mm), and image acquisition was conducted using NIS-Elements software (Nikon). .. For the 3D reconstruction and rendering, IMARIS 9.6 (Bitplane AG) software was used.

    Article Title: In vivo programming of adult pericytes aids axon regeneration by providing cellular bridges for SCI repair.
    Article Snippet: 872 873 Calcium imaging 874 The membrane-permeable calcium indicator Fluo-4 AM (F14201, Invitrogen) was added 875 to the cell culture medium (final concentration: 5 μM) and kept at 36.5°C in the cell culture 876 incubator for 45-60 minutes. .. The coverslip was then transferred to a submerged imaging 877 chamber mounted on a Nikon upright confocal microscope. ..

    Article Title: Early life ACE2 inhibition leads to long term anxiety-like behaviour in rats by disrupting the amygdala.
    Article Snippet: .. To assess the number of microglia, Iba1 images were taken on an upright confocal microscope (Nikon Eclipse 90i, Melville, NY, USA) using a 20x objective, with a 488 nm laser with a 515/30 filter set to detect fluorescently labelled Iba-1, and a 405 nm laser to detect DAPI. .. For morphological analysis of microglia, we used Imaris (Bitplane) in association with MATLAB R2017b programming language software, version 9.3.0.7.

    Article Title: MSL10 is a high-sensitivity mechanosensor in the tactile sense of the Venus flytrap.
    Article Snippet: .. The sections were observed under an upright confocal microscope (A1R, Nikon) equipped with a 4× objective lens (Plan Fluor 4X, Nikon). ..

    Article Title: A novel protein-preserving passive tissue clearing approach using sodium cholate and urea for whole-organ imaging
    Article Snippet: .. The imaging was performed using an upright confocal microscope (C2Si, Nikon) with a Plan-Apochromat 10× objective lens (numerical aperture (NA) 0.5 and working distance (WD) 5.5 mm), and image acquisition was conducted using NIS-Elements software (Nikon). .. For the 3D reconstruction and rendering, IMARIS 9.6 (Bitplane AG) software was used.

    Imaging:

    Article Title: A novel protein-preserving passive tissue clearing approach using sodium cholate and urea for whole-organ imaging.
    Article Snippet: .. The imaging was performed using an upright confocal microscope (C2Si, Nikon) with a Plan-Apochromat 10× objective lens (numerical aperture (NA) 0.5 and working distance (WD) 5.5 mm), and image acquisition was conducted using NIS-Elements software (Nikon). .. For the 3D reconstruction and rendering, IMARIS 9.6 (Bitplane AG) software was used.

    Article Title: In vivo programming of adult pericytes aids axon regeneration by providing cellular bridges for SCI repair.
    Article Snippet: 872 873 Calcium imaging 874 The membrane-permeable calcium indicator Fluo-4 AM (F14201, Invitrogen) was added 875 to the cell culture medium (final concentration: 5 μM) and kept at 36.5°C in the cell culture 876 incubator for 45-60 minutes. .. The coverslip was then transferred to a submerged imaging 877 chamber mounted on a Nikon upright confocal microscope. ..

    Article Title: A novel protein-preserving passive tissue clearing approach using sodium cholate and urea for whole-organ imaging
    Article Snippet: .. The imaging was performed using an upright confocal microscope (C2Si, Nikon) with a Plan-Apochromat 10× objective lens (numerical aperture (NA) 0.5 and working distance (WD) 5.5 mm), and image acquisition was conducted using NIS-Elements software (Nikon). .. For the 3D reconstruction and rendering, IMARIS 9.6 (Bitplane AG) software was used.

    Software:

    Article Title: A novel protein-preserving passive tissue clearing approach using sodium cholate and urea for whole-organ imaging.
    Article Snippet: .. The imaging was performed using an upright confocal microscope (C2Si, Nikon) with a Plan-Apochromat 10× objective lens (numerical aperture (NA) 0.5 and working distance (WD) 5.5 mm), and image acquisition was conducted using NIS-Elements software (Nikon). .. For the 3D reconstruction and rendering, IMARIS 9.6 (Bitplane AG) software was used.

    Article Title: A novel protein-preserving passive tissue clearing approach using sodium cholate and urea for whole-organ imaging
    Article Snippet: .. The imaging was performed using an upright confocal microscope (C2Si, Nikon) with a Plan-Apochromat 10× objective lens (numerical aperture (NA) 0.5 and working distance (WD) 5.5 mm), and image acquisition was conducted using NIS-Elements software (Nikon). .. For the 3D reconstruction and rendering, IMARIS 9.6 (Bitplane AG) software was used.



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    ( A to H ) Migration and deformation of endogenous murine neutrophils 2 hours after N. meningitidis local skin injection. (A) <t>Spinning-disk</t> confocal images of skin capillaries with neutrophils indicated by arrowheads. BSA, bovine serum albumin. (B) Capillary segments with different diameters. (C) Frequency distribution of capillary diameters ( n = 139) and round neutrophils in large vessels ( n = 67). (D) Neutrophil confinement in capillaries. Neutrophils shown in orange. (E) Montage of 30 cells pseudocolored by the value of their AR. (F) Individual mean cell AR and (G) mean cell speed ( n = 61 cells). (H) Scatterplot comparing instantaneous speed and instantaneous cell AR ( n = 679 time points from 61 cells). ( I to L ) Mature mNeus migrating in microchannels of different widths. (I) Representative widefield microscopy images of individual mNeus (Hoechst, magenta) in each channel size. (J) Distribution of mean cell AR, n > 150 cells per channel size, and (K) mean cell speed, n > 250 cells per channel size. (L) Scatterplot comparing instantaneous speed and instantaneous cell AR of mNeus in 7-, 5-, and 3-μm channel widths. Data are one representative of three independent experiments; n = 2189 (7 μm), n = 2876 (5 μm), and n = 2137 (3 μm). Data are pooled from [(C) and (F) to (H)] three or (K) five independent experiments. Data are displayed as [(F) and (G)] median by a line or [(C), (J), and (K)] frequency distributions (percentage) with indicated means ± SD. Each dot represents one cell. Data were analyzed by [(H) and (L)] Spearman correlation test (two-tailed). Scale bars, [(B), (D), and (I)] 5 μm, (E) 10 μm, and (A) 20 μm.
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    Image Search Results


    ( A to H ) Migration and deformation of endogenous murine neutrophils 2 hours after N. meningitidis local skin injection. (A) Spinning-disk confocal images of skin capillaries with neutrophils indicated by arrowheads. BSA, bovine serum albumin. (B) Capillary segments with different diameters. (C) Frequency distribution of capillary diameters ( n = 139) and round neutrophils in large vessels ( n = 67). (D) Neutrophil confinement in capillaries. Neutrophils shown in orange. (E) Montage of 30 cells pseudocolored by the value of their AR. (F) Individual mean cell AR and (G) mean cell speed ( n = 61 cells). (H) Scatterplot comparing instantaneous speed and instantaneous cell AR ( n = 679 time points from 61 cells). ( I to L ) Mature mNeus migrating in microchannels of different widths. (I) Representative widefield microscopy images of individual mNeus (Hoechst, magenta) in each channel size. (J) Distribution of mean cell AR, n > 150 cells per channel size, and (K) mean cell speed, n > 250 cells per channel size. (L) Scatterplot comparing instantaneous speed and instantaneous cell AR of mNeus in 7-, 5-, and 3-μm channel widths. Data are one representative of three independent experiments; n = 2189 (7 μm), n = 2876 (5 μm), and n = 2137 (3 μm). Data are pooled from [(C) and (F) to (H)] three or (K) five independent experiments. Data are displayed as [(F) and (G)] median by a line or [(C), (J), and (K)] frequency distributions (percentage) with indicated means ± SD. Each dot represents one cell. Data were analyzed by [(H) and (L)] Spearman correlation test (two-tailed). Scale bars, [(B), (D), and (I)] 5 μm, (E) 10 μm, and (A) 20 μm.

    Journal: Science immunology

    Article Title: Adaptive motility enables neutrophils to rapidly navigate confined capillaries

    doi: 10.1126/sciimmunol.aea1426

    Figure Lengend Snippet: ( A to H ) Migration and deformation of endogenous murine neutrophils 2 hours after N. meningitidis local skin injection. (A) Spinning-disk confocal images of skin capillaries with neutrophils indicated by arrowheads. BSA, bovine serum albumin. (B) Capillary segments with different diameters. (C) Frequency distribution of capillary diameters ( n = 139) and round neutrophils in large vessels ( n = 67). (D) Neutrophil confinement in capillaries. Neutrophils shown in orange. (E) Montage of 30 cells pseudocolored by the value of their AR. (F) Individual mean cell AR and (G) mean cell speed ( n = 61 cells). (H) Scatterplot comparing instantaneous speed and instantaneous cell AR ( n = 679 time points from 61 cells). ( I to L ) Mature mNeus migrating in microchannels of different widths. (I) Representative widefield microscopy images of individual mNeus (Hoechst, magenta) in each channel size. (J) Distribution of mean cell AR, n > 150 cells per channel size, and (K) mean cell speed, n > 250 cells per channel size. (L) Scatterplot comparing instantaneous speed and instantaneous cell AR of mNeus in 7-, 5-, and 3-μm channel widths. Data are one representative of three independent experiments; n = 2189 (7 μm), n = 2876 (5 μm), and n = 2137 (3 μm). Data are pooled from [(C) and (F) to (H)] three or (K) five independent experiments. Data are displayed as [(F) and (G)] median by a line or [(C), (J), and (K)] frequency distributions (percentage) with indicated means ± SD. Each dot represents one cell. Data were analyzed by [(H) and (L)] Spearman correlation test (two-tailed). Scale bars, [(B), (D), and (I)] 5 μm, (E) 10 μm, and (A) 20 μm.

    Article Snippet: Image acquisitions were done using an upright spinning-disk confocal microscope (DM6 FS; Leica) equipped with a motorized stage (Z Deck; Prior Scientific).

    Techniques: Migration, In Vivo, In Vitro, Injection, Spinning Disk, Confocal, Widefield, Microscopy, Two Tailed Test